MitoTam bromide, hydrobromide|cas:1634624-73-9|西域试剂

MitoTam bromide, hydrobromide,98.24%

产品编号：Bellancom-126222| CAS NO：1634624-73-9| 分子式：C₅₂H₆₀Br₂NOP| 分子量：905.82

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货号	价格	库存与货期
Bellancom-126222	￥5000.00	杭州北京（现货）
Bellancom-126222	￥8500.00	杭州北京（现货）
Bellancom-126222	￥18000.00	杭州北京（现货）
Bellancom-126222	￥30000.00	杭州北京（现货）
Bellancom-126222	￥46000.00	杭州北京（现货）

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MitoTam bromide, hydrobromide

产品介绍

MitoTam bromide, hydrobromide 他莫昔芬衍生物，它是一种电子传递链 (ETC) 抑制剂，抑制衰老细胞中的线粒体膜电位变化并影响线粒体形态。MitoTam bromide, hydrobromide 是一种有效的抗癌剂，可以抑制乳腺癌细胞中呼吸复合物 (CI-respiration) 和超复合物 (SCs) 的形成。

生物活性

MitoTam bromide, hydrobromide, a Tamoxifen derivative, is an electron transport chain (ETC) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology. MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells.

体外研究

MitoTam (0.5 μM-56 μM; 24 hours) kills breast cancer cell Lines and nonmalignant cells with an IC₅₀ range from 0.65 μM to 55.9 μM.
MitoTam (2.5 μM; 2-24 hours) results in stronger activation of the apoptotic pathway in MCF7 Her2 high cells compared with mock MCF7 cells.
MitoTam (0.05 μM-1 μM; 3 days) causes a concentration-dependent induction of apoptosis in breast cancer cells, while there was no effect for non-malignant breast epithelial cells

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Breast Cancer Cell Lines: BT474, MCF7, MCF7 Her2^high, MCF7 Her2^low, MDA-MB-231, MDA-MB-436, MDA-MB-453, SK-BR-3, T47D; NeuTL cells; Nonmalignant Cells: A014578, H9c2 cells
Concentration:	0.5 μM-56 μM
Incubation Time:	24 hours
Result:	Killed breast cancer cells MCF7, MCF7 Her2^high, MCF7 Her2^low with IC₅₀ values of 1.25 μM, 0.65 μM and 1.45 μM respectively.

Western Blot Analysis

Cell Line:	MCF7 mock cells, MCF7 Her2^high cells
Concentration:	2.5 μM
Incubation Time:	2 hours, 4 hours, 8 hours, 16 hours, 24 hours
Result:	Revealed accelerated cleavage of procaspase-9, Parp1/2 and proapoptotic Bax, decreased the antiapoptotic Bcl-2 protein in Her2high cells.

Apoptosis Analysis

Cell Line:	MCF-7 cells, 4T1 cells and MCF-10a cells
Concentration:	0.05 μM-1 μM
Incubation Time:	3 days
Result:	Resulted in apoptosis in MCF7 and 4T1 cells.

体内研究
(In Vivo)

MitoTam (intraperitoneal injection; 2 μg/g; once a week; 4 weeks) decreases β-gal staining of lungs from MitoTam-treated mice, accompaning by a inhibition in the expression of senescence markers p16Ink4a, p21waf1 and PAI comparing control mice sup>.
MitoTam (intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks) inhibits growth of syngeneic tumors by 80%.
MitoTam (intraperitoneal injection; 0.25 μmol/mouse; twice a week; 2 weeks) slows down the growth of MCF7 mock tumors and stops tumor progression after two doses; suppresses Her2^high carcinomas decreased threefold from the original size with complete disappearance.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	18-month-old or 2-month-old FVB/N mice
Dosage:	2 μg/g
Administration:	Intraperitoneal injection; 2 μg/g; once a week; 4 weeks
Result:	Eliminated senescent cells also in vivo.

Animal Model:	FVB/N c-neu mouse
Dosage:	0.54 μmol/mouse
Administration:	Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks
Result:	Suppressed Her2^high breast carcinomas.

Animal Model:	Balb/c nude mice with MCF7 mock or MCF7 Her2^high cells
Dosage:	0.25 μmol/mouse/dose
Administration:	Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks
Result:	Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2^high carcinomas decreased.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	18-month-old or 2-month-old FVB/N mice
Dosage:	2 μg/g
Administration:	Intraperitoneal injection; 2 μg/g; once a week; 4 weeks
Result:	Eliminated senescent cells also in vivo.

Animal Model:	FVB/N c-neu mouse
Dosage:	0.54 μmol/mouse
Administration:	Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks
Result:	Suppressed Her2^high breast carcinomas.

Animal Model:	Balb/c nude mice with MCF7 mock or MCF7 Her2^high cells
Dosage:	0.25 μmol/mouse/dose
Administration:	Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks
Result:	Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2^high carcinomas decreased.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	18-month-old or 2-month-old FVB/N mice
Dosage:	2 μg/g
Administration:	Intraperitoneal injection; 2 μg/g; once a week; 4 weeks
Result:	Eliminated senescent cells also in vivo.

Animal Model:	FVB/N c-neu mouse
Dosage:	0.54 μmol/mouse
Administration:	Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks
Result:	Suppressed Her2^high breast carcinomas.

Animal Model:	Balb/c nude mice with MCF7 mock or MCF7 Her2^high cells
Dosage:	0.25 μmol/mouse/dose
Administration:	Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks
Result:	Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2^high carcinomas decreased.

性状

Solid

溶解性数据

In Vitro:

DMSO : 240 mg/mL (264.95 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.1040 mL	5.5199 mL	11.0397 mL
5 mM	0.2208 mL	1.1040 mL	2.2079 mL
10 mM	0.1104 mL	0.5520 mL	1.1040 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)

参考文献