Guanabenz hydrochloride|cas:23113-43-1|西域试剂

Guanabenz hydrochloride,99.95%

产品编号：Bellancom-12724A| CAS NO：23113-43-1| 分子式：C₈H₉Cl₃N₄| 分子量：267.54

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-12724A	￥430.00	杭州北京（现货）
Bellancom-12724A	￥800.00	杭州北京（现货）
Bellancom-12724A	￥1500.00	杭州北京（现货）

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Guanabenz hydrochloride

产品介绍

Guanabenz hydrochloride 是一种 α-2 肾上腺素受体激动剂，具有口服活性。Guanabenz hydrochloride 具有抗高血压和抗寄生虫活性。Guanabenz hydrochloride 干扰内质网应激信号传导，对心肌细胞具有保护作用。Guanabenz hydrochloride也被用于高血压的研究。

生物活性

Guanabenz hydrochloride is an orally active α-2-adrenoceptor agonist. Guanabenz hydrochloride has antihypertensive effect and antiparasitic activity. Guanabenz hydrochloride interferes ER stress-signalling and has protective effects in cardiac myocytes. Guanabenz hydrochloride also is used for the research of high blood pressure.

体外研究

Guanabenz hydrochloride (0.5-50 μM, 24 h) is treated with increasing concentrations for 24 hours not affect cell viability.
Guanabenz hydrochloride (0.5-50 μM, 24 h) alone not affects the UPR targets, neither on mRNA or protein level nor the phosphorylation status of eIF2a. Guanabenz also not induces GADD34 or the constitutively active form CReP.
Guanabenz hydrochloride (0.5-50 μM, 24 h) alone not induces ER stress in neonatal rat cardiomyocytes.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Neonatal rat cardiac myocytes (NRCM)
Concentration:	0.5-50 μM
Incubation Time:	24 h
Result:	Did not affect cell survival.

Western Blot Analysis

Cell Line:	Neonatal rat cardiac myocytes (NRCM)
Concentration:	0.5-50 μM
Incubation Time:	24 h
Result:	Increased the levels of low panel concentration-dependent UPR targets proteins.

RT-PCR

Cell Line:	Neonatal rat cardiac myocytes (NRCM)
Concentration:	0.5-50 μM
Incubation Time:	24 h
Result:	Did not affect levels of UPR targets.

体内研究
(In Vivo)

Guanabenz hydrochloride (5 mg/kg/day; i.p.; for 3 weeks) can reproducibly reduce brain cyst burden.
Guanabenz hydrochloride (5 mg /kg/d, i.p., oral; 10 mg/kg/d, gavage; for 3 weeks) reverses Toxoplasma-induced hyperactivity in latently infected mice.
Guanabenz hydrochloride (100 and 320 μg/kg and 1 mg/kg, i.v., over a period of 5 min at intervals of 40 min) reduces sympathetic outflow, heart rate and blood pressure in debuffered cats.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/cJ mice
Dosage:	5 mg/kg
Administration:	5 mg/kg/day; i.p. ; for 3 weeks
Result:	Reduced the latent brain cysts in both male and female BALB/cJ mice.

Animal Model:	BALB/cJ mice
Dosage:	5 mg/kg; 10 mg/kg
Administration:	5 mg /kg/d, i.p., oral; 10 mg/kg/d, gavage; for 3 weeks
Result:	Reversed parasite-induced hyperactivity to near-baseline levels.

Animal Model:	Cats
Dosage:	100 and 320 μg/kg and 1 mg/kg
Administration:	100 and 320 μg/kg and 1 mg/kg, i.v., over a period of 5 min at intervals of 40 min
Result:	Declined markedly blood pressure and nerve activity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/cJ mice
Dosage:	5 mg/kg
Administration:	5 mg/kg/day; i.p. ; for 3 weeks
Result:	Reduced the latent brain cysts in both male and female BALB/cJ mice.

Animal Model:	BALB/cJ mice
Dosage:	5 mg/kg; 10 mg/kg
Administration:	5 mg /kg/d, i.p., oral; 10 mg/kg/d, gavage; for 3 weeks
Result:	Reversed parasite-induced hyperactivity to near-baseline levels.

Animal Model:	Cats
Dosage:	100 and 320 μg/kg and 1 mg/kg
Administration:	100 and 320 μg/kg and 1 mg/kg, i.v., over a period of 5 min at intervals of 40 min
Result:	Declined markedly blood pressure and nerve activity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/cJ mice
Dosage:	5 mg/kg
Administration:	5 mg/kg/day; i.p. ; for 3 weeks
Result:	Reduced the latent brain cysts in both male and female BALB/cJ mice.

Animal Model:	BALB/cJ mice
Dosage:	5 mg/kg; 10 mg/kg
Administration:	5 mg /kg/d, i.p., oral; 10 mg/kg/d, gavage; for 3 weeks
Result:	Reversed parasite-induced hyperactivity to near-baseline levels.

Animal Model:	Cats
Dosage:	100 and 320 μg/kg and 1 mg/kg
Administration:	100 and 320 μg/kg and 1 mg/kg, i.v., over a period of 5 min at intervals of 40 min
Result:	Declined markedly blood pressure and nerve activity.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (373.78 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.7378 mL	18.6888 mL	37.3776 mL
5 mM	0.7476 mL	3.7378 mL	7.4755 mL
10 mM	0.3738 mL	1.8689 mL	3.7378 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (9.34 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.34 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.34 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.34 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.34 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.34 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。