6,2',4'-Trimethoxyflavone|cas:720675-74-1|西域试剂

6,2',4'-Trimethoxyflavone,98.09%

产品编号：Bellancom-103220| CAS NO：720675-74-1| 分子式：C₁₈H₁₆O₅| 分子量：312.32

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-103220	￥600.00	杭州北京（现货）
Bellancom-103220	￥1000.00	杭州北京（现货）
Bellancom-103220	￥2000.00	杭州北京（现货）
Bellancom-103220	￥3200.00	杭州北京（现货）
Bellancom-103220	￥5000.00	杭州北京（现货）

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6,2',4'-Trimethoxyflavone

产品介绍

6,2',4'-Trimethoxyflavone 是一种有效的芳香烃受体 (AHR) 拮抗剂。(AHR) 抑制AHR介导的基因诱导。

生物活性

6,2',4'-Trimethoxyflavone is a potent aryl hydrocarbon receptor (AHR) antagonist. 6,2',4'-Trimethoxyflavone represses AHR-mediated gene induction.

体外研究

6,2',4'-trimethoxyflavone (TMF) as an AHR ligand that possesses the characteristics of an antagonist having the capacity to compete with agonists, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin and benzo[a]pyrene, thus effectively inhibiting AHR-mediated transactivation of a heterologous reporter and endogenous targets, e.g., CYP1A1, independent of cell lineage or species. Furthermore, TMF displays superior action by virtue of having no partial agonist activity, in contrast to other documented antagonists, e.g., alpha-napthoflavone, which are partial weak agonists. TMF also exhibits no species or promoter dependence with regard to AHR antagonism.
6,2',4'-Trimethoxyflavone (0-100 μM; 72 hours) shows an inhibitory activity of TNF-⍺ production in THP-1 cells, with IC₅₀ of 2.38 μM. 6,2',4'-Trimethoxyflavone shows an inhibitory activity of TNF-α production in B16-F10 cells with IC₅₀ of 1.32 μM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	THP-1 cells, B16-F10 cells
Concentration:	0-100 μM
Incubation Time:	72 hours
Result:	Showed inhibitory activity of TNF-⍺ production in THP-1 cells and B16-F10 cells.

体内研究
(In Vivo)

6,2',4'-trimethoxyflavone-treated (5 mg/kg/day; i.p.) WT mice shows significantly decreased infarct volume, improved sensorimotor, and nonspatial working memory functions compared with their respective controls.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 wild-type (WT) mice, AHRcKO mice
Dosage:	5 mg/kg/day
Administration:	I.p.
Result:	Both TMF-treated and AHRcKO mice attenuated acute cerebral infarction and functional impairments.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 wild-type (WT) mice, AHRcKO mice
Dosage:	5 mg/kg/day
Administration:	I.p.
Result:	Both TMF-treated and AHRcKO mice attenuated acute cerebral infarction and functional impairments.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 wild-type (WT) mice, AHRcKO mice
Dosage:	5 mg/kg/day
Administration:	I.p.
Result:	Both TMF-treated and AHRcKO mice attenuated acute cerebral infarction and functional impairments.

性状

Solid

溶解性数据

In Vitro:

DMSO : 5 mg/mL (16.01 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2018 mL	16.0092 mL	32.0184 mL
5 mM	0.6404 mL	3.2018 mL	6.4037 mL
10 mM	0.3202 mL	1.6009 mL	3.2018 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 0.5 mg/mL (1.60 mM); Clear solution

此方案可获得 ≥ 0.5 mg/mL (1.60 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 5.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 0.5 mg/mL (1.60 mM); Clear solution

此方案可获得 ≥ 0.5 mg/mL (1.60 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 5.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明