Bafilomycin C1 巴菲霉素C1|cas:88979-61-7|西域试剂

Bafilomycin C1 巴菲霉素C1,99.0%

产品编号：Bellancom-130173| CAS NO：88979-61-7| 分子式：C₃₉H₆₀O₁₂| 分子量：720.89

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-130173		￥3500.00	杭州北京（现货）
Bellancom-130173		￥9800.00	杭州北京（现货）

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Bafilomycin C1 巴菲霉素C1

产品介绍

Bafilomycin C1 是从链霉菌中分离得到的大环内酯类抗生素 (antibiotic)。Bafilomycin C1 是一种有效的、特异性的、可逆的 vacuolar-type H⁺-ATPases 抑制剂。Bafilomycin C1 抑制革兰氏阳性菌 (bacteria) 和真菌 (fungi) 的生长。Bafilomycin C1 可诱导细胞凋亡 (apoptosis)，可用于肝细胞癌 (HCC) 的研究。

生物活性

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H⁺-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC).

体外研究

Bafilomycin C1 (0.33-10 μM; 6 days) inhibits the growth and proliferation of SMMC7721 and HepG2 cells in a timeand dose-dependent manner.
Bafilomycin C1 (0.33-3.3 μM; 24 hours) decreases cyclin D3, cyclin E1, CDK2, CDK4, and CDK6 expression in both mRNA and protein expression in SMMC7721 cells.
Bafilomycin C1 (3.3-10 μM; 24 hours) causes morphological alterations and increases the population of apoptotic cells by Hoechst 33258 (HY-15558) staining compared to vehicle.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	SMMC7721 cell and HepG2 cell
Concentration:	0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721 1.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time:	6 days
Result:	Retarded the cell growth.

Western Blot Analysis

Cell Line:	SMMC7721 cells
Concentration:	3.3 μM
Incubation Time:	24 hours
Result:	Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis

Cell Line:	SMMC7721 and HepG2 cells
Concentration:	3.3 μM; 10 μM
Incubation Time:	24 hours
Result:	Induced apoptosis in SMMC7721 and HepG2 cells.

体内研究
(In Vivo)

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×10⁶ cells/100 μL)
Dosage:	0.2 mg/kg
Administration:	Subcutaneous injection; 20 days
Result:	Suppressed tumor growth of SMMC7721 tumor xenografts.

体内研究

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×10⁶ cells/100 μL)
Dosage:	0.2 mg/kg
Administration:	Subcutaneous injection; 20 days
Result:	Suppressed tumor growth of SMMC7721 tumor xenografts.

体内研究

Bafilomycin C1 (subcutaneous injection; 0.2 mg/kg; 20 days) retards the tumor growth without apparent adverse reactions or side effects in nude mice model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×10⁶ cells/100 μL)
Dosage:	0.2 mg/kg
Administration:	Subcutaneous injection; 20 days
Result:	Suppressed tumor growth of SMMC7721 tumor xenografts.