KEA1-97|cas:2138882-71-8|西域试剂

KEA1-97,99.66%

产品编号：Bellancom-114982| CAS NO：2138882-71-8| 分子式：C₁₅H₉Cl₂FN₄| 分子量：335.16

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-114982	￥700.00	杭州北京（现货）
Bellancom-114982	￥1100.00	杭州北京（现货）
Bellancom-114982	￥3800.00	杭州北京（现货）
Bellancom-114982	￥6100.00	杭州北京（现货）

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KEA1-97

产品介绍

KEA1-97 是一种选择性Thioredoxin-caspase 3相互作用干扰剂 (IC₅₀=10 μM)。KEA1-97 在不影响 thioredoxin 活性的情况下, 破坏 thioredoxin 与 caspase 3 的相互作用, 激活 caspases, 诱导细胞 apoptosis。

生物活性

KEA1-97 is a selective Thioredoxin-caspase 3 interaction disruptor (IC₅₀=10 μM). KEA1-97 disrupts the interaction of thioredoxin with caspase 3, activates caspases, and induces apoptosis without affecting thioredoxin activity.

体外研究

KEA1-97 (100 μM; 231MFP cells) impaires thioredoxin pulldown of caspase 3.
KEA1-97 (10 μM; 48 hours; 231MFP cells) impaires 231MFP serum-free cell proliferation.
KEA1-97 (10 μM; 0~12 hours; 231MFP cells) activates caspase 3/7 and induces apoptotic cell death.
KEA1-97 (231MFP cells) resistants to survival and proliferation impairments.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	231MFP cells
Concentration:	100 μM
Incubation Time:
Result:	Impaired thioredoxin pulldown of caspase 3.

Cell Proliferation Assay

Cell Line:	231MFP cells
Concentration:	10 μM
Incubation Time:	48 h
Result:	Impaired 231MFP serum-free cell proliferation.

Apoptosis Analysis

Cell Line:	231MFP cells
Concentration:	10 μM
Incubation Time:	0~12 h
Result:	Activated caspase 3/7 and induced apoptotic cell death.

体内研究
(In Vivo)

KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Immune-deficient SCID mice
Dosage:	5 mg/kg
Administration:	I.p.; 50 days
Result:	Attenuated tumor xenograft growth.

体内研究

KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Immune-deficient SCID mice
Dosage:	5 mg/kg
Administration:	I.p.; 50 days
Result:	Attenuated tumor xenograft growth.

体内研究

KEA1-97 (5 mg/kg; i.p.; 50 days) attenuates tumor xenograft growth.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Immune-deficient SCID mice
Dosage:	5 mg/kg
Administration:	I.p.; 50 days
Result:	Attenuated tumor xenograft growth.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (298.36 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9836 mL	14.9182 mL	29.8365 mL
5 mM	0.5967 mL	2.9836 mL	5.9673 mL
10 mM	0.2984 mL	1.4918 mL	2.9836 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.46 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。