Rohinitib|cas:1139253-73-8|西域试剂

Rohinitib,99.40%

产品编号：Bellancom-148422| CAS NO：1139253-73-8| 分子式：C₂₉H₃₁NO₈| 分子量：521.56

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-148422		￥4500.00	杭州北京（现货）
Bellancom-148422		￥8000.00	杭州北京（现货）

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Rohinitib

产品介绍

Rohinitib 是一种有效的以及特异性的 eIF4A 抑制剂。Rohinitib 诱导急性髓系白血病 (AML) 细胞系的凋亡，并减轻在 AML 异种移植模型小鼠的白血病负担。Rohinitib 可用于 AML 的研究。

生物活性

Rohinitib is a potent and specific eIF4A inhibitor. Rohinitib induces cell apoptosis of acute myeloid leukemia (AML) cell lines and reduces the leukemia burden of AML xenograft model. Rohinitib can be used for the research of AML.

体外研究

Rohinitib (6.25-50 nM; 72 h) induces cell apoptosis of AML cell lines and FLT3-ITD-positive AML cell lines.
Primary AML cells is more sensitive to Rohinitib (25 nM; 72 h) than normal bone marrow (BM) and FLT3-ITD-positive cells is more sensitive to Rohinitib than FLT3 wild-type AML cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis

Cell Line:	AML cell lines
Concentration:	6.25, 12.5, 25 and 50 nM
Incubation Time:	72 h
Result:	Dose-dependently induced apoptosis of MOLM-13, MOLM-14, MV4;11, OCI-AML3, THP-1, HL-60, Kasumi-1 and NB4 cell lines. And significantly induced cell apoptosis of FLT3-ITD, FLT3-ITD-expressing murine Ba/F3 and human OCI-AML3 cells.

体内研究
(In Vivo)

Rohinitib (0.75 and 1.0 mg/kg; s.c. once daily for 5 consecutive days until mice get moribund) shows anti-AML effects in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female NSG mice with AML xenografts generated by intravenous injections of MOLM-13 cells
Dosage:	0.75 and 1.0 mg/kg
Administration:	Subcutaneous injection; 0.75 and 1.0 mg/kg once daily 5 days a week until mice get moribund
Result:	Significantly reduced the leukemia burden, circulating and BM leukemic human CD45⁺ cells. Dose-dependently prolonged the survival rate of mice.

体内研究

Rohinitib (0.75 and 1.0 mg/kg; s.c. once daily for 5 consecutive days until mice get moribund) shows anti-AML effects in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female NSG mice with AML xenografts generated by intravenous injections of MOLM-13 cells
Dosage:	0.75 and 1.0 mg/kg
Administration:	Subcutaneous injection; 0.75 and 1.0 mg/kg once daily 5 days a week until mice get moribund
Result:	Significantly reduced the leukemia burden, circulating and BM leukemic human CD45⁺ cells. Dose-dependently prolonged the survival rate of mice.

体内研究

Rohinitib (0.75 and 1.0 mg/kg; s.c. once daily for 5 consecutive days until mice get moribund) shows anti-AML effects in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female NSG mice with AML xenografts generated by intravenous injections of MOLM-13 cells
Dosage:	0.75 and 1.0 mg/kg
Administration:	Subcutaneous injection; 0.75 and 1.0 mg/kg once daily 5 days a week until mice get moribund
Result:	Significantly reduced the leukemia burden, circulating and BM leukemic human CD45⁺ cells. Dose-dependently prolonged the survival rate of mice.

性状

Solid

溶解性数据

In Vitro:

DMSO : 200 mg/mL (383.46 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9173 mL	9.5866 mL	19.1732 mL
5 mM	0.3835 mL	1.9173 mL	3.8346 mL
10 mM	0.1917 mL	0.9587 mL	1.9173 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 5 mg/mL (9.59 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (9.59 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 5 mg/mL (9.59 mM); Clear solution

此方案可获得 ≥ 5 mg/mL (9.59 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 50.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。