Lupiwighteone 黄羽扇豆魏特酮; 8-prenylgenistein|cas:104691-86-3|西域试剂

Lupiwighteone 黄羽扇豆魏特酮; 8-prenylgenistein,98.63%

产品编号：Bellancom-N3354| CAS NO：104691-86-3| 分子式：C₂₀H₁₈O₅| 分子量：338.35

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N3354		￥4100.00	杭州北京（现货）
Bellancom-N3354		￥7300.00	杭州北京（现货）

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Lupiwighteone 黄羽扇豆魏特酮; 8-prenylgenistein

产品介绍

Lupiwighteone 是一种广泛存在于野生植物中的异黄酮，具有抗氧化、抗菌和抗癌作用。Lupiwighteone 通过抑制 PI3K/Akt/mTOR 通路，诱导人乳腺癌细胞 caspase 依赖性和非依赖性凋亡 (apoptosis)。

生物活性

Lupiwighteone is an isoflavone present widely in wild-growing plants, with antioxidant, antimicrobial and anticancer effects. Lupiwighteone induces caspase-dependent and -independent apoptosis on human breast cancer cells via inhibiting PI3K/Akt/mTOR pathway.

体外研究

Lupiwighteone (2-100 μM; 72 hours) shows cytotoxicity towards various cell lines, especially on DU-145 cells and SGC-7901 cells with IC₅₀s of 23.7 μM and 21 μM, respectively.
Lupiwighteone (20-60 μM; 48 hours) induces (cell cycle arrest in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces cells apoptosis.
Lupiwighteone (20-60 μM; 48 hours) decreases the cell cycle-related protein expressions in a dose-dependent manner in DU-145 cells.
Lupiwighteone (20-60 μM; 48 hours) induces a dose-dependent increase in ROS production.
Lupiwighteone up-regulates of cytochrome c and caspase-3, and subsequent cleavage of PARP-1 and down-regulates of the p-Akt/Akt ratio and VEGF expression, suggests the activation of mitochondria-based intrinsic apoptosis in DU-145 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	DU-145 cells, C4-2 cells, SGC-7901 cells, Hela cells, HUVEC, MCF-7 cells, CNE cells, Vero
Concentration:	2 μM, 5 μM, 25 μM, 50 μM, 100 μM
Incubation Time:	72 hours
Result:	Had inhibition on the survival of many cancer cell lines and HUVEC.

Cell Cycle Analysis

Cell Line:	DU-145 cells
Concentration:	20 μM, 40 μM, 60 μM
Incubation Time:	48 hours
Result:	Induced cell cycle arrest.

Apoptosis Analysis

Cell Line:	DU-145 cells
Concentration:	20 μM, 40 μM, 60 μM
Incubation Time:	48 hours
Result:	Induced apoptosis.

Western Blot Analysis

Cell Line:	DU-145 cells
Concentration:	20 μM, 40 μM, 60 μM
Incubation Time:	48 hours
Result:	Decreased CDK1, 2, 4, 6, cyclinD1, and cyclinB1 protein expression in a dose-dependent manner.

分子量

338.35

性状

Solid

Formula

C₂₀H₁₈O₅

CAS 号

104691-86-3

中文名称

黄羽扇豆魏特酮

结构分类

Flavonoids
Isoflavones

Phenols
Polyphenols

初始来源

Plants
other families

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (295.55 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9555 mL	14.7776 mL	29.5552 mL
5 mM	0.5911 mL	2.9555 mL	5.9110 mL
10 mM	0.2956 mL	1.4778 mL	2.9555 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.39 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.39 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。