Norartocarpetin|cas:520-30-9|西域试剂

Norartocarpetin,98.92%

产品编号：Bellancom-N10503| CAS NO：520-30-9| 分子式：C₁₅H₁₀O₆| 分子量：286.24

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-N10503	￥1400.00	杭州北京（现货）
Bellancom-N10503	￥3500.00	杭州北京（现货）
Bellancom-N10503	￥5800.00	杭州北京（现货）
Bellancom-N10503	￥11000.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

Norartocarpetin

产品介绍

Norartocarpetin 是一种酪氨酸酶抑制剂。Norartocarpetin 具有较强的酪氨酸酶抑制活性，其抑制活性 IC₅₀ 值为 0.47 μM。Norartocarpetin 作为一种抗褐变剂可用于食品体系的研究。Norartocarpetin 对肺癌细胞 (NCI-H460) 也具有显著的抗癌活性，其 IC₅₀ 值为 22 μM。Norartocarpetin 的抗增殖作用是通过靶向 Ras/Raf/MAPK 信号通路、线粒体介导的细胞凋亡、S 期细胞周期阻滞和抑制细胞迁移和侵袭实现的。

生物活性

Norartocarpetin is a tyrosinase inhibitor. Norartocarpetin has strong tyrosinase inhibitory activity with an IC₅₀ value of 0.47 μM. Norartocarpetin as an antibrowning agent can be used for the research of food systems. Norartocarpetin also has a significant anticancer activity in lung carcinoma cells (NCI-H460) with an IC₅₀ value of 22 μM. Norartocarpetin has antiproliferative effects are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial mediated apoptosis, S-phase cell cycle arrest and suppression of cell migration and invasion in human lung carcinoma cells.

体外研究

Norartocarpetin has strong mushroom tyrosinase inhibitory activity with an IC₅₀ value of 0.47 μM.
Norartocarpetin (0-100 μM; 48 h) leds to dose-dependent cytotoxic effects in NCI-H460 cells and MRC-9 cells with IC₅₀ values of 22 μM and 85 μM, respectively.
Norartocarpetin (0, 11, 22, and 44 μM; 24 h) led to blockade of Ras/Raf/MAPK signalling pathway.
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) induced the apoptosis of the human lung carcinoma cells (NCI-H460).
Norartocarpetin (0, 11, 22, and 44 μM) leds to S-phase cell cycle arrest.
Norartocarpetin (22 μM; 24 h) leds to dose-dependent suppression of cell invasion.
Norartocarpetin (0, 11, 22, and 44 μM, 24 h) leds to significant inhibition of cell migration.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	MRC-9 (normal fibroblast lung cells) and NCI-H460 (human lung cancer cells)
Concentration:	0, 3.12, 6.25, 12.5, 25, 50, and 100 μM (first dissolved in DMSO at 1.0 mg/mL and then diluted to different concentrations using DMSO, 30 μL, 20min)
Incubation Time:	48 h
Result:	Repressed the viability of NCI-H460 cells in a dose-dependent manner.

Western Blot Analysis

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0, 11, 22, and 44 μM
Incubation Time:	24 h
Result:	Significantly reduced the expression of p-RAS, p-RAF and p-P38 and unchanged the expressions of RAS, RAF and P38.

Apoptosis Analysis

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0. 11, 22 and 44 μM
Incubation Time:	24 h
Result:	Led to dose-dependent apoptosis and induced DNA damage.

Cell Cycle Analysis

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0, 11, 22, and 44 μM
Incubation Time:
Result:	Showed increase of S-phase cells and the percentage of S-phase NCI-H460 LC cells was 30%, 40%, 55% and 70% at 0, 11, 22 and 44 μM concentrations, respectively.

Cell Invasion AssayMRC-9 and NCI-H460 cells

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	22 μM
Incubation Time:	24 h
Result:	Significantly reducted (from 100% to about 40%) in the invasion of NCI-H460 human LC cells.

Cell Migration Assay

Cell Line:	MRC-9 and NCI-H460 cells
Concentration:	0, 11, 22, and 44 μM
Incubation Time:	24 h
Result:	Decreased remarkably the cell invasion in NCI-H460 LC cells in a concentration- dependent manner.

分子量

286.24

性状

Solid

Formula

C₁₅H₁₀O₆

CAS 号

520-30-9

结构分类

Flavonoids
Flavones

初始来源

Plants
Moraceae

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (349.36 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.4936 mL	17.4679 mL	34.9357 mL
5 mM	0.6987 mL	3.4936 mL	6.9871 mL
10 mM	0.3494 mL	1.7468 mL	3.4936 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

参考文献