RRD-251|cas:72214-67-6|西域试剂

RRD-251,99.94%

产品编号：Bellancom-117737A| CAS NO：72214-67-6| 分子式：C₈H₉Cl₃N₂S| 分子量：271.59

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货号	价格	库存与货期
Bellancom-117737A	￥500.00	杭州北京（现货）
Bellancom-117737A	￥800.00	杭州北京（现货）
Bellancom-117737A	￥1600.00	杭州北京（现货）
Bellancom-117737A	￥2500.00	杭州北京（现货）
Bellancom-117737A	￥4000.00	杭州北京（现货）

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RRD-251

产品介绍

RRD-251 是一种视网膜母细胞瘤肿瘤抑制蛋白 (Rb)-Raf-1相互作用 (Rb-Raf-1 interaction) 的抑制剂，具有高效的抗增殖、抗血管生成和抗肿瘤活性。

生物活性

RRD-251 is an inhibitor of retinoblastoma tumor suppressor protein (Rb)-Raf-1 interaction, with potent anti-proliferative, anti-angiogenic and anti-tumor activities.

体外研究

RRD-251 (10-50 μM; 24 hours) inhibits melanoma growth in-vitro.
RRD-251 (50 μM; 2 hours) inhibits Rb-Raf-1 interaction and Rb phosphorylation in non-small cell lung cancer cells.
RRD-251 induces apoptosis (50 μM; 18 hours) and cell cycle arrest (20-50 μM; 4 hours).
RRD-251 alters the expression of cell cycle and apoptosis regulatory protein.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	SK-MEL-28 cells , SK-MEL-5 cells, SK-MEL-2 cells
Concentration:	10 μM, 20 μM, 50 μM
Incubation Time:	24 hours
Result:	Inhibited melanoma growth in-vitro.

Western Blot Analysis

Cell Line:	SK-MEL-28 cells , SK-MEL-5 cells, SK-MEL-2 cells
Concentration:	50 μM
Incubation Time:	2 hours
Result:	Showed the depletion of phosphorylated-Rb in RRD-251 treated cells.

Apoptosis Analysis

Cell Line:	SK-MEL-28 cells , SK-MEL-5 cells, SK-MEL-2 cells
Concentration:	50 μM
Incubation Time:	18 hours
Result:	Induced apoptosis.

Cell Cycle Analysis

Cell Line:	SK-MEL-28 cells , SK-MEL-5 cells
Concentration:	20 μM, 50 μM
Incubation Time:	4 hours
Result:	Resulted in a dose dependent inhibition of cell cycle progression in SK-MEL-28 and SK-MEL-5 cells, respectively.

体内研究
(In Vivo)

RRD-251 (50 mg/kg; i.p.; q.o.d; for 14 days) has anti-cancer activities in vivo on melanomas.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-wk-old female athymic nude mice, with SK-ME-28 xenograft
Dosage:	50 mg/kg
Administration:	Intraperitoneal administration, qod, for 14 days
Result:	Inhibits the growth of SK-ME-28 xenograft in nude mice.

体内研究

RRD-251 (50 mg/kg; i.p.; q.o.d; for 14 days) has anti-cancer activities in vivo on melanomas.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-wk-old female athymic nude mice, with SK-ME-28 xenograft
Dosage:	50 mg/kg
Administration:	Intraperitoneal administration, qod, for 14 days
Result:	Inhibits the growth of SK-ME-28 xenograft in nude mice.

体内研究

RRD-251 (50 mg/kg; i.p.; q.o.d; for 14 days) has anti-cancer activities in vivo on melanomas.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-wk-old female athymic nude mice, with SK-ME-28 xenograft
Dosage:	50 mg/kg
Administration:	Intraperitoneal administration, qod, for 14 days
Result:	Inhibits the growth of SK-ME-28 xenograft in nude mice.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (460.25 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6820 mL	18.4101 mL	36.8202 mL
5 mM	0.7364 mL	3.6820 mL	7.3640 mL
10 mM	0.3682 mL	1.8410 mL	3.6820 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

参考文献