Harringtonolide|cas:64761-48-4|西域试剂

Harringtonolide,98.0%

产品编号：Bellancom-N10335| CAS NO：64761-48-4| 分子式：C₁₉H₁₈O₄| 分子量：310.34

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N10335		￥19200.00	杭州北京（现货）

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Harringtonolide

产品介绍

Harringtonolide 是一种有效的 RACK1 抑制剂，在 A375 细胞中 IC₅₀=39.66 μM。Harringtonolide 通过影响 FAK 和 RACK1 的相互作用来抑制上皮-间充质转化 (EMT) 过程和细胞增殖。Harringtonolide 具有抑制植物生长、抗病毒、抗炎、抗增殖活性。

生物活性

Harringtonolide is a potent RACK1 inhibitor (IC₅₀=39.66 μM in A375 cells). Harringtonolide inhibits the epithelial-mesenchymal transition (EMT) process and cell proliferation by affecting the interaction between FAK and RACK1. Harringtonolide has plant growth inhibitory, antiviral, anti-inflammatory, and antiproliferation activities.

体外研究

Harringtonolide (0-50 μM; 24 hours) exhibits good antiproliferation activity in A375 cells with IC₅₀ of 39.66 μM.
Harringtonolide (0-20 μM; 1 hour) restrains the proteolysis of RACK1 by Pronase, and protects temperature-dependent degradation of RACK1.
Harringtonolide (0-4 μM; 24 hours) suppresses the phosphorylation of FAK dose-dependently and inhibits Src and STAT3, the downstream proteins of FAK.
Harringtonolide (0-4 μM; 24 hours) dose-dependently inhibits the RACK1–FAK interaction in A375 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	A375 melanoma cells
Concentration:	0-50 μM
Incubation Time:	24 hours
Result:	Showed good antiproliferation activity with IC₅₀ of 39.66 μM.

Western Blot Analysis

Cell Line:	A375 melanoma cells
Concentration:	0, 0.5, 1, 2, 4 μM
Incubation Time:	24 hours
Result:	Suppressed the phosphorylation of FAK dose-dependently and inhibited Src and STAT3, the downstream proteins of FAK.