WM-3835|cas:2229025-70-9|西域试剂

WM-3835,98.06%

产品编号：Bellancom-134901| CAS NO：2229025-70-9| 分子式：C₂₀H₁₇FN₂O₄S| 分子量：400.42

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-134901	￥1000.00	杭州北京（现货）
Bellancom-134901	￥1700.00	杭州北京（现货）
Bellancom-134901	￥3500.00	杭州北京（现货）
Bellancom-134901	￥5500.00	杭州北京（现货）
Bellancom-134901	￥9500.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

WM-3835

产品介绍

WM-3835 是一种有效的，高特异性 HBO1 (KAT7 or MYST2) 抑制剂，可直接与 HBO1 33 的乙酰辅酶 A 结合位点结合 WM-3835 激活细胞凋亡，同时抑制骨肉瘤 (OS) 细胞的增殖，迁移和侵袭。WM-3835 具有抗肿瘤活性，并有效抑制小鼠中骨肉瘤异种移植物的生长。

生物活性

WM-3835 is a potent and high-specific HBO1 (KAT7 or MYST2) inhibitor and binds directly to the acetyl-CoA binding site of HBO1 33. WM-3835 activates apoptosis while inhibits osteosarcoma (OS) cell proliferation, migration and invasion. WM-3835 has antitumor activity and potently inhibits pOS-1 xenograft growth in mice.

体外研究

WM-3835 (1-25 uM; 24-96 hours) inhibits pOS-1 cell viability in a concentration-dependent manner.
WM-3835 (5 uM; 72 h) activates cell apoptosis and significantly increases TUNEL-positive nuclei in pOS-1 cells.
WM-3835 (5 µM; 24 hours) downregulates MYLK-HOXA9 mRNA expression in pOS-1 cells.
WM-3835 (1-25 uM) suppresses H4K12ac-H3K14ac in a dose-dependent manner. WM-3835 does not alter the expressions of HBO1 protein and total H3, H4 histones.
WM-3835 (5 µM) fails to induce apoptosis and reduction of viability in HBO1-KO pOS-1 cells, koHBO1-1 and koHBO1-2, HBO1-low human osteoblasts.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Primary human OS (pOS-1) cells
Concentration:	1, 5, 10, 25 uM
Incubation Time:	24, 48, 72, 96 hours
Result:	Inhibited pOS-1 cell viability in a concentration-dependent manner. Exerted a significant anti-survival activity at least 48 h, displaying a time-dependent manner.

Apoptosis Analysis

Cell Line:	pOS-1 cells
Concentration:	5 uM
Incubation Time:	72 hours
Result:	Activated cell apoptosis and significantly increases TUNEL-positive nuclei.

RT-PCR

Cell Line:	pOS-1 cells
Concentration:	5 uM
Incubation Time:	24 hours
Result:	Downregulated MYLK-HOXA9 mRNA expression.

体内研究
(In Vivo)

WM-3835 (10 mg/kg/day; ip; for21 days) potently inhibits pOS-1 xenograft growth in SCID mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID mice (18-19 g, female) with pOS1 cells
Dosage:	10 mg/kg
Administration:	IP; daily; for 21 days
Result:	Potently inhibited pOS-1 xenograft growth.

体内研究

WM-3835 (10 mg/kg/day; ip; for21 days) potently inhibits pOS-1 xenograft growth in SCID mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID mice (18-19 g, female) with pOS1 cells
Dosage:	10 mg/kg
Administration:	IP; daily; for 21 days
Result:	Potently inhibited pOS-1 xenograft growth.

体内研究

WM-3835 (10 mg/kg/day; ip; for21 days) potently inhibits pOS-1 xenograft growth in SCID mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	SCID mice (18-19 g, female) with pOS1 cells
Dosage:	10 mg/kg
Administration:	IP; daily; for 21 days
Result:	Potently inhibited pOS-1 xenograft growth.

性状

Solid

溶解性数据

In Vitro:

DMSO : 250 mg/mL (624.34 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4974 mL	12.4869 mL	24.9738 mL
5 mM	0.4995 mL	2.4974 mL	4.9948 mL
10 mM	0.2497 mL	1.2487 mL	2.4974 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.19 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.19 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.19 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.19 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。