ASR-490|cas:2690312-67-3|西域试剂

ASR-490,99.05%

产品编号：Bellancom-144899| CAS NO：2690312-67-3| 分子式：C₃₄H₄₁NO₇| 分子量：575.69

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-144899		￥6500.00	杭州北京（现货）
Bellancom-144899		￥10500.00	杭州北京（现货）

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ASR-490

产品介绍

ASR-490 通过下调 Notch1 信号传导降低 HCT116 和 SW620 细胞的活力。ASR-490 克服 Notch1 的过表达并抑制 HCT/Notch1 转染子的生长。ASR-490 在异种移植小鼠中抑制对照 (pCMV/HCT116) 和 Notch1/HCT116 的肿瘤生长。

生物活性

ASR-490 reduces the viability of HCT116 and SW620 cells by downregulating Notch1 signaling. ASR-490 overcomes Notch1 overexpression and inhibits the growth of HCT/Notch1 transfectants. ASR-490 inhibits the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

体外研究

ASR-490 (0-1.6 µM; 24 h, 48 h) reduces the viability of HCT116 and SW620 cells in 24h (IC₅₀ =750 nM in HCT116 cells, IC₅₀ =1.2 µM in SW620 cells) and 48h (IC₅₀ =600 nM in HCT116 cells, IC₅₀ =850 nM in SW620 cells). ASR-490 (750 nM in HCT116 cells and 1.2 µM in SW620 cells, 24 h) shows apoptotic cell death and upregulation of the proapoptotic markers Bax and cleaved PARP Expression; inhibits the capability of colorectal cancer cells. ASR-490 (HCT116 cells; 750 nM in 24 h, 600 nM in 48 h) overcomes Notch1 overexpression and inhibits the growth of HCT/Notch1 transfectants.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	HCT116, SW620 cells
Concentration:	0-1.6 µM
Incubation Time:	24 h, 48 h
Result:	Reduced the viability of HCT116 and SW620 cells in 24h (IC₅₀ =750 nM in HCT116 cells, IC₅₀ =1.2 µM in SW620 cells) and 48h (IC₅₀ =600 nM in HCT116 cells, IC₅₀ =850 nM in SW620 cells).

Apoptosis Analysis

Cell Line:	HCT116, SW620 cells
Concentration:	750 nM in HCT116 cells and 1.2 µM in SW620 cells
Incubation Time:	24 h
Result:	Showed apoptotic cell death and upregulation the proapoptotic markers Bax and cleaved PARP Expression, inhibited the capability of colorectal cancer cells.

Cell Proliferation Assay

Cell Line:	HCT116 cells
Concentration:	750 nM in 24 h, 600 nM in 48 h
Incubation Time:	24 h, 48 h
Result:	Overcome Notch1 overexpression and inhibited the growth of HCT/Notch1 transfectants.

体内研究
(In Vivo)

ASR-490 (5 mg/kg; i.p.; thrice a week for 4 weeks) inhibits the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to 8-week-old BALB/c athymic nude mice (nu/nu) (pCMV/HCT116 and Notch1/HCT116 (C4) xenografts).
Dosage:	5 mg/kg
Administration:	i.p., thrice a week for 4 weeks
Result:	Inhibited the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

体内研究

ASR-490 (5 mg/kg; i.p.; thrice a week for 4 weeks) inhibits the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to 8-week-old BALB/c athymic nude mice (nu/nu) (pCMV/HCT116 and Notch1/HCT116 (C4) xenografts).
Dosage:	5 mg/kg
Administration:	i.p., thrice a week for 4 weeks
Result:	Inhibited the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

体内研究

ASR-490 (5 mg/kg; i.p.; thrice a week for 4 weeks) inhibits the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to 8-week-old BALB/c athymic nude mice (nu/nu) (pCMV/HCT116 and Notch1/HCT116 (C4) xenografts).
Dosage:	5 mg/kg
Administration:	i.p., thrice a week for 4 weeks
Result:	Inhibited the tumor growth in control (pCMV/HCT116) and Notch1/HCT116 in xenotransplanted mice.

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (43.43 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7370 mL	8.6852 mL	17.3705 mL
5 mM	0.3474 mL	1.7370 mL	3.4741 mL
10 mM	0.1737 mL	0.8685 mL	1.7370 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (4.34 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.34 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (4.34 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.34 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (4.34 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.34 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。