Biotin-PEG3-C3-NH2 is a PEG-based PROTAC linker, with NH₂ functional group, that can be used in the synthesis of PROTACs.

PROTAC linkers exert function for connecting the two different ligands that make up PROTACs, the two ligands are E3 ubiquitin ligase and the target protein, respectively.
PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
PROTAC can significantly attenuate tumor progression in tumor xenografts.
Biotin-PEG3-C3-NH2 (compound 11) (0-2 mM; 3 h) combinded with protein G (compound 6) displays fluorescence under streptavidin probes, Biotin-PEG3-C3-NH2 is a powerful tool for the high-throughput analysis of protein-small molecule interaction and profiling enzyme activities in a microarrays constructed by surface derivatization method.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562.  [Content Brief]

  . Park S, et al. One-step, aid-mediated method for modification of glass surfaces with N-hydroxysuccinimide esters and its application to the construction of microarrays for studies of biomolecular interactions. Bioconjug Chem. 2010 Jul 21;21(7):1246-53.  [Content Brief]