Liquiritigenin-treated murine osteoblastic MC3T3-E1 cells shows an increased alkaline phosphatase activity and enhances phosphorylation of Smad1/5 compared with untreated cells. Moreover, liquiritigenin inhibits osteoclast differentiation, its bone-resorption activity through slightly decreases the phosphorylation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, and inhibitor of nuclear factor kappa B-α ; however, the phosphorylation of Akt and p38 slightly increase in bone marrow-derived osteoclasts. The expression levels of the osteoclast marker proteins nuclear factor of activated T-cell cytoplasmic-1, Src, and cathepsin K diminish. Liquiritigenin is shown to exert the following pharmacological effects: increased cell growth, increased alkaline phosphatase activity, promotion of collagen synthesis, and the mineralization of osteoblastic MC3T3-E1 cells Liquiritigenin inhibits serum-induced HIF-1α and VEGF expression via the AKT/mTOR-p70S6K signaling pathway in HeLa cells.

Liquiritigenin inhibits the growth of tumors in nude mice originating from human cervical cancer cell line HeLa cells, and reduces angiogenesis in a dose dependent manner. Liquiritigenin effectively reduces the levels of pro-inflammatory cytokines and the expressions of p-p65NF-κB and p-IκBα. Furthermore, liquiritigenin preconditioning could down-regulate the immobility time in tail suspension test (TST), forced swimming test (FST) and up-regulate BDNF and TrkB contents in hippocampus. Thus, liquiritigenin has antidepressant activity that might be attributed to its anti-inflammatory property and BDNF/TrkB signaling pathway

• Cell lines: Murine osteoblastic MC3T3-E1 cells
• Concentrations: 0-100 μM
• Incubation Time: 72 h
• Method: Cells seeded in 96-well cell culture plates are incubated with the Cell Counting Kit-8 for 1 h, and then the absorbance at 450nm is measured with a microplate reader.

• Cell lines: Murine osteoblastic MC3T3-E1 cells
• Concentrations: 0-100 μM
• Incubation Time: 72 h
• Method: Cells seeded in 96-well cell culture plates are incubated with the Cell Counting Kit-8 for 1 h, and then the absorbance at 450nm is measured with a microplate reader.

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