The Amyloid β-peptide (1-40) (rat)-treated rats require longer to find the platform hidden under the water. In the visible platform test, the latency for each group is similar (20.56±11.31 s in control and 14.68±9.97 s in Amyloid β-peptide (1-40) (rat) group, p=0.304). Among these tested proteins, 6 ones are significantly up-regulated and 7 ones significantly down-regulated in Amyloid β-peptide (1-40) (rat) group comparing with the control. Tubulin β chain is significantly down-regulated in Amyloid β-peptide (1-40) (rat) group (p=0.014) when compare with control. Similar changes are also observed for ATP synthase β subunit (p=0.010) and synapsin Ib between Amyloid β-peptide (1-40) (rat) and control groups, but not significantly for synapsin Ib (p=0.295).

在该研究中使用体重为200至220g的8周龄雄性Sprague-Dawley大鼠（n = 14）。将大鼠圈养在塑料笼中，随意提供食物和水，并在室温（21至23℃）下保持12小时光照/黑暗循环。将14只大鼠随机分为两组：（1）注射Milli-Q水的大鼠作为对照组;（2）注射纤维性神经毒性淀粉样蛋白β-肽（1-40）（大鼠）作为淀粉样β蛋白肽（1-40）（大鼠）组。以0.5μL/ min的流速注射纤维状淀粉样蛋白β-肽（1-40）（大鼠）或Milli-Q水（2μL）。注射后，将针在缓慢提取前保持原位5分钟。第二次注射也在相对半球的相同坐标处施用。通过降压被动回避试验和Morris水迷宫试验评估动物的行为表现。