EWP 815|cas:20231-01-0|西域试剂

EWP 815,98.25%

产品编号：Bellancom-148001| CAS NO：20231-01-0| 分子式：C₁₂H₂₂N₄S₄| 分子量：350.59

EWP 815是一种二硫仑类似物，是Ins（1,4）P2磷酸酶和Ins（1,4,5）P3 5-磷酸酶的有效抑制剂。EWP 815还抑制多巴胺β-羟化酶活性。

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-148001	￥850.00	杭州北京（现货）
Bellancom-148001	￥1400.00	杭州北京（现货）
Bellancom-148001	￥2800.00	杭州北京（现货）
Bellancom-148001	￥4500.00	杭州北京（现货）
Bellancom-148001	￥7200.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

EWP 815

产品介绍

EWP 815 是一种二硫类似物，是 Ins(1,4)P₂ 磷酸酶和 Ins(1,4,5)P₃ 5-磷酸酶的有效抑制剂。EWP 815 也参与抑制体内多巴胺酶 β-羟化酶活性。

生物活性

EWP 815 is a disulfiram analogue, is a potent inhibitor of Ins(1,4)P₂ phosphatase and Ins(1,4,5)P₃ 5-phosphatase. EWP 815 also inhibits enzyme dopamine β-hydroxylase activity.

体外研究

EWP 815 inhibits thyrotropin-releasing hormone (TRH; 0.03 mM)-stimulated inositol phospholipid breakdown without affecting basal breakdown rates in prelabelled GH₃ cells with IC₅₀s of 83 mM (soluble enzymes) and 71 mM (particulate enzymes), respectively.
EWP 815 (30 μM; 1 h) exerts higher inhibition on Ins(1,4)P₂ phosphatase rather than Ins(1,4,5)P₃ 5-phosphatase with the mean [³H]Ins(1,4)P₂/mean [³H]InsP recovery ratio of 2.6.
EWP 815 (6, 8 μM; 10 min) inhibits dephosphorylation of [³H]Ins(1,4,5)P₃ 5-phosphatase in particulate and soluble fractions with IC₅₀s of 6 μM and 8 μM, respectively.
EWP 815 (3-300 μM; 30 min) suppresses thyrotropin-releasing hormone (TRH)-stimulated (100 nM) inositol phospholipid production by 30% (100 μM) and 1.8% (300 μM), respectively, without affecting Ins(1,4,5)P₃ binding.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

EWP 815 (50 mg/kg; i.p.; 30 min before killed) inhibits the enzyme dopamine β-hydroxylase activity in mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female mice (20 g)
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; 30 min before killed
Result:	Intraperitoneal injection; 30 min before killedInhibited β-hydroxylase of ³H-α-methyldopamine. Reduced the amount of ³H-α-Me-NA by 12%.

体内研究

EWP 815 (50 mg/kg; i.p.; 30 min before killed) inhibits the enzyme dopamine β-hydroxylase activity in mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female mice (20 g)
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; 30 min before killed
Result:	Intraperitoneal injection; 30 min before killedInhibited β-hydroxylase of ³H-α-methyldopamine. Reduced the amount of ³H-α-Me-NA by 12%.

性状

Solid

溶解性数据

In Vitro:

DMSO : 50 mg/mL (142.62 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8523 mL	14.2617 mL	28.5233 mL
5 mM	0.5705 mL	2.8523 mL	5.7047 mL
10 mM	0.2852 mL	1.4262 mL	2.8523 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.25 mg/mL (3.57 mM); Clear solution

此方案可获得 ≥ 1.25 mg/mL (3.57 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 1.25 mg/mL (3.57 mM); Clear solution

此方案可获得 ≥ 1.25 mg/mL (3.57 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

参考文献

. Fowler CJ, et al. Inhibition of inositol 1,4,5-trisphosphate 5-phosphatase by micromolar concentrations of disulfiram and its analogues. Biochem J. 1993 Feb 1;289 ( Pt 3)(Pt 3):853-9. [Content Brief]

. Carlsson A, et al. On the beta-hydroxylation of (+-)-alpha-methyldopamine in vivo. Eur J Pharmacol. 1968 Dec;5(1):85-92. [Content Brief]

化学品安全说明书(MSDS)

下载MSDS

质检证书(COA)

产品编号(Item Number)

批号(Lot Number)

安全声明 (欧洲)	S24/25
海关编码	2933599090

上游产品 1
5712-49-2
下游产品 0

EWP 815,98.25%

EWP 815

化学品安全说明书(MSDS)

质检证书(COA)

在线咨询