Z-FY-CHO Z-Phe-Tyr-CHO|cas:167498-29-5|西域试剂

Z-FY-CHO Z-Phe-Tyr-CHO,95.0%

产品编号：Bellancom-128140| CAS NO：167498-29-5| 分子式：C₂₆H₂₆N₂O₅| 分子量：446.50

Z-FY-CHO（Z-Phe-Tyr-CHO）是一种有效且特异的组织蛋白酶L（CTSL）抑制剂。

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货号	价格	库存与货期
Bellancom-128140	￥1000.00	杭州北京（现货）
Bellancom-128140	￥1600.00	杭州北京（现货）
Bellancom-128140	￥5800.00	杭州北京（现货）
Bellancom-128140	￥9500.00	杭州北京（现货）

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Z-FY-CHO Z-Phe-Tyr-CHO

产品介绍

Z-FY-CHO (Z-Phe-Tyr-CHO) 是一种有效且特异性的组织蛋白酶 L (CTSL) 抑制剂。

生物活性

Z-FY-CHO (Z-Phe-Tyr-CHO) is a potent and specific cathepsin L (CTSL) inhibitor.

体外研究

Z-FY-CHO (10 µM, for 1 h) pretreatment alleviated cell death induced by 6-OHDA. Z-FY-CHO inhibits the increase in CTSL protein expression in 6-OHDA-treated SH-SY5Y cells. Treatment with Z-FY-CHO caused more LC3-II and less P62 expression in SH-SY5Y cells treated with 6-OHDA, indicating enhancing autophagy activity, and Z-FY-CHO blocks activation of caspase-3 and PARP.
Treatment with the specific cathepsin L inhibitor Z-FY-CHO (10 μM) or transfection with cathepsin L shRNA significantly increased the radiosensitivity of U251 cells. Both suppression and knockdown of cathepsin L in U251 cells increased irradiation-induced DNA damage and G2/M phase cell cycle arrest. Both suppression and knockdown of cathepsin L in U251 cells also increased irradiation-induced apoptosis, as shown by the increased levels of Bax and decreased levels of Bcl-2.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

Intraperitoneal administration of Z-FY-CHO (2.5-10 mg/kg) for 4 weeks suppresses bone weight loss dose dependently in the ovariectomized mouse, experimental model of osteoporosis. Z-FY-CHO acts as a bone resorption suppressor through the inhibition of collagen degradation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (223.96 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2396 mL	11.1982 mL	22.3964 mL
5 mM	0.4479 mL	2.2396 mL	4.4793 mL
10 mM	0.2240 mL	1.1198 mL	2.2396 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1.25 mg/mL (2.80 mM); Clear solution

此方案可获得 ≥ 1.25 mg/mL (2.80 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 1.25 mg/mL (2.80 mM); Suspended solution; Need ultrasonic

此方案可获得 1.25 mg/mL (2.80 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 1.25 mg/mL (2.80 mM); Clear solution

此方案可获得 ≥ 1.25 mg/mL (2.80 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

参考文献

. Lingyun Li, et al. Activated cathepsin L is associated with the switch from autophagy to apoptotic death of SH-SY5Y cells exposed to 6-hydroxydopamine. Biochem Biophys Res Commun. 2016 Feb 12;470(3):579-585. [Content Brief]

. Qing-qing Zhang, et al. Cathepsin L suppression increases the radiosensitivity of human glioma U251 cells via G2/M cell cycle arrest and DNA damage. Acta Pharmacol Sin. 2015 Sep;36(9):1113-25. [Content Brief]

. J T Woo, et al. Suppressive effect of N-(benzyloxycarbonyl)-L-phenylalanyl-L-tyrosinal on bone resorption in vitro and in vivo. Eur J Pharmacol. 1996 Apr 4;300(1-2):131-5. [Content Brief]

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产品编号(Item Number)

批号(Lot Number)

Z-FY-CHO Z-Phe-Tyr-CHO,95.0%

Z-FY-CHO Z-Phe-Tyr-CHO

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