Deoxypodophyllotoxin 脱氧鬼臼毒素|cas:19186-35-7|西域试剂

Deoxypodophyllotoxin 脱氧鬼臼毒素,99.86%

产品编号：Bellancom-N2500| CAS NO：19186-35-7| 分子式：C₂₂H₂₂O₇| 分子量：398.41

Deoxypodophyllotoxin (DPT)，鬼臼毒素的衍生物，是一种木酚素，从 Sinopodophullumhexandrum (小檗科)的根茎分离，具有强效抗有丝分裂和抗病毒特性。Deoxypodophyllotoxin 作为靶向微管的药物，不仅抗有丝分裂剂而且作为有效的血管生成抑制剂，在肿瘤学中具有重大影响。Deoxypodophyllotoxin 诱导细胞自噬 (autophagy) 和细胞凋亡 (apoptosis)。Deoxypodophyllotoxin 引起 DRG 神经元细胞内 Ca2+ 浓度增加。

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N2500		￥2900.00	杭州北京（现货）
Bellancom-N2500		￥5300.00	杭州北京（现货）

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Deoxypodophyllotoxin 脱氧鬼臼毒素

产品介绍

Deoxypodophyllotoxin (DPT)，鬼臼毒素的衍生物，是一种木酚素，从 Anthriscus sylvestris分离，具有强效抗有丝分裂和抗病毒特性。Deoxypodophyllotoxin 作为靶向微管的活性分子，不仅抗有丝分裂剂而且作为有效的血管生成抑制剂，在肿瘤学中具有重大影响。Deoxypodophyllotoxin 诱导细胞自噬 (autophagy) 和细胞凋亡 (apoptosis)。Deoxypodophyllotoxin 引起 DRG 神经元细胞内 Ca²⁺ 浓度增加。

生物活性

Deoxypodophyllotoxin (DPT), a derivative of podophyllotoxin, is a lignan with potent antimitotic, anti-inflammatory and antiviral properties isolated from Anthriscus sylvestris. Deoxypodophyllotoxin, targets the microtubule, has a major impact in oncology not only as anti-mitotics but also as potent inhibitors of angiogenesis. Deoxypodophyllotoxin induces cell autophagy and apoptosis. Deoxypodophyllotoxin evokes increase of intracellular Ca²⁺ concentrations in DRG neurons.

体外研究

Deoxypodophyllotoxin (25-75 nM; 6-48 hours) increases the percentage of early apoptotic cell population from 2.05 to 5.62 and 18.49% for 24 h and 48 h, respectively.
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) treats SGC-7901 cells arrested in G2/M phase in time- and dose- dependent manners.
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) results in a remarkably time- and dose-dependent decrease in Cdc2 and Cdc25C expression levels and increases cyclin B1 within 6h, decreases PARP, Bcl-2 and caspase-3 activity.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis

Cell Line:	SGC-7901 cells
Concentration:	25, 50, 75 nM
Incubation Time:	6, 12, 24, 48 hours
Result:	Induced apoptosis in SGC-7901 Cells.

Cell Cycle Analysis

Cell Line:	SGC-7901 cells
Concentration:	25, 50, 75 nM
Incubation Time:	6, 12, 24, 48 hours
Result:	Induced G2/M cell cycle arrest in SGC-7901 Cells

Western Blot Analysis

Cell Line:	SGC-7901 cells
Concentration:	25, 50, 75 nM
Incubation Time:	6, 12, 24, 48 hours
Result:	Altered the expression of cyclin B1, Cdc2,Cdc25C, p-PARP, Bcl-2 and p-caspase-3 proteins.

体内研究
(In Vivo)

Deoxypodophyllotoxin (intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days) suppresses the tumors in a dose-dependent manner, the growth of tumors is inhibited by 22.19%, 47.91% and 50.93% with DPT at 5, 10 and 20 mg/kg, respectively.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft model of gastric cancer in nude mice with SGC-7901 cells
Dosage:	5, 10, and 20 mg/kg
Administration:	Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days
Result:	Inhibited the growth of gastric cancer tumors.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft model of gastric cancer in nude mice with SGC-7901 cells
Dosage:	5, 10, and 20 mg/kg
Administration:	Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days
Result:	Inhibited the growth of gastric cancer tumors.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft model of gastric cancer in nude mice with SGC-7901 cells
Dosage:	5, 10, and 20 mg/kg
Administration:	Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days
Result:	Inhibited the growth of gastric cancer tumors.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (251.00 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.5100 mL	12.5499 mL	25.0998 mL
5 mM	0.5020 mL	2.5100 mL	5.0200 mL
10 mM	0.2510 mL	1.2550 mL	2.5100 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.27 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.27 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.27 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

参考文献

. Wang YR, et al. Deoxypodophyllotoxin induces G2/M cell cycle arrest and apoptosis in SGC-7901 cells and inhibits tumor growth in vivo. Molecules. 2015 Jan 20;20(1):1661-75. [Content Brief]

. Kim SH, et al. Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells. Pharmacol Rep. 2017 Oct;69(5):878-884. [Content Brief]

. Xu P, et al. Pharmacological effect of deoxypodophyllotoxin: a medicinal agent of plant origin, on mammalian neurons. Neurotoxicology. 2010 Dec;31(6):680-6. [Content Brief]

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Deoxypodophyllotoxin 脱氧鬼臼毒素,99.86%

Deoxypodophyllotoxin 脱氧鬼臼毒素

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